Journal: PeerJ
Article Title: Ischemic postconditioning influences electron transport chain protein turnover in Langendorff-perfused rat hearts
doi: 10.7717/peerj.1706
Figure Lengend Snippet: Langendorff hearts were treated with 5-HD (A) or IPo (B) after I/R insult. Total mitochondrial protein samples were separated using linear IPG strips (24 cm, pH 5–8), and stained with silver nitrate. (C) Protein spots with differential parts per million (PPM) value were annotated with numbers in an image synthesized by PDQuest software. Protein in spot 3002 (detected as ATPA by MASCOT peptide mass fingerprint (PMF) and indicated with a black arrow) was detected as a differential protein by PDQuest. (D) Enlarged 2DE gel images and 3D view showing the 5-HD- and IPo-dependent protein expression changes of ATPA.
Article Snippet: Software contained PDQuest advanced 8.0 2DE analysis software (Bio-Rad, Hercules, CA, USA), data explorer, mass spectrometry analysis software, Mascot Distiller mass spectrometry signal peak recognition software, Mascot peptide mass fingerprinting database query software (Applied Biosystem; Life Technologies, Carlsbad, CA, USA); Mascot TOF/MS database equery software (Matrix Science, London, UK) and Odyssey infrared imaging analysis software (LI-COR, Lincoln, NE, USA).
Techniques: Staining, Synthesized, Software, Expressing